Cottrell et al Version 0.2 Contents 1 Getting Started 3 1.1 Layout of the liquid handling system 3 1.2 Layout of the in situ sample stick 4 1.3 Layout of the pump 4 2.1 Sample loading 6 2.1.1 Loading Vessel 1 6 2.1.2 Loading Vessel 2 6 2.2 Evacuation of the system 6 2.2.1 Removing air from Vessel 1 7 2.2.2 Removing air from Vessel 2 7 2.2.3 Removing air from Vessel 3 7 2.2.4 Evacuation of the sample cell 7 2.3 Freeze-Pump-Thaw cycle 8 2.3.1 Sample in Vessel 1 8 2.3.2 Sample in Vessel 2 8 2.3.3 S